Development of SSR Markers forPsychotria homalosperma(Rubiaceae) and Cross-Amplification in Four Other Species
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چکیده
منابع مشابه
Cross-species amplification of eucalyptus SSR markers in Casuarinaceae
Cross-species amplification of Simple Sequence Repeats (SSRs) loci is considered as a cost-effective approach for developing microsatellite markers for new species. We examined the transferability of eucalyptus SSR loci to the economically important members of the Casuarinaceae family. Ten primer pairs targeting (GA)n, (CTT)n, (TGA)n and (GAA)n motifs were screened in Allocasuarina littoralis, ...
متن کاملNuclear and chloroplast SSR markers in Paeonia delavayi (Paeoniaceae) and cross-species amplification in P. ludlowii.
PREMISE OF THE STUDY Microsatellite primers were developed for Paeonia delavayi and P. ludlowii (Paeoniaceae) to study their population genetics and phytogeography. METHODS AND RESULTS Nine polymorphic nuclear microsatellite loci were isolated from an enriched library of P. delavayi and primers were designed. The number of alleles per locus ranged from two to 16; the observed and expected het...
متن کاملDevelopment of Simple Sequence Repeats (SSR) Markers in Setaria italica (Poaceae) and Cross-Amplification in Related Species
Foxtail millet is one of the world's oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and tr...
متن کاملDevelopment of EST-SSR markers in Barringtonia racemosa (Lecythidaceae) and cross-amplification in related species1
PREMISE OF THE STUDY Microsatellite markers were identified and characterized to study the genetic diversity and structure of Barringtonia racemosa (Lecythidaceae). METHODS AND RESULTS Based on the transcriptome data of B. racemosa, 30 primer pairs were initially designed and tested, of which 15 were successfully amplified and displayed clear polymorphisms across the 43 individuals from three...
متن کاملDevelopment of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener1
PREMISE OF THE STUDY To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for ...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2016
ISSN: 2168-0450
DOI: 10.3732/apps.1500133